Structures Of The E. Coli Ribosome With Ribosome Recycling Factor And Antibiotics

Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. Aminoglycosides are widely used antibiotics that, among their effects, inhibit ribosome recycling. I will present x‐ray crystal structures of the E. coli 70S ribosome with RRF and/or aminoglycosides bound that provide a structural explanation for aminoglycoside inhibition of ribosome recycling. I will also present structures of the ribosome with the antibiotic spectinomycin, which inhibits mRNA and tRNA translocation on the ribosome.