Cyclooxygenases (COX) play a key role in the regulation of renal arterial tone, but not in glomerular function,of mouse with 5/6 nephrectomy (LB725)

Objectives. Hyperfusion induces shear stress, leading to macrovascular endothelial dysfunction. However, effects of hyperfusion on glomerular endothelial cells (microvascular) remain unclear. Methods. C57/BL6 mice were randomly grouped into sham-operated (2K), 1 kidney removal (1K) and 5/6 nephroectomy groups (5/6K-off). Renal artery and glomerulus were collected for Western blotting, polymerase chain reaction and immunohistochemistry (IHC) assay. Results. Four weeks after the surgery, arterial blood pressure was comparable among the three groups. Compared with 2K and 1K groups, 5/6K-off mice had significantly higher levels of renal blood flow. Consistently, relaxations to acetylcholine were blunted in renal arteries, but not in aortae, of 5/6K-off mice. Indomethacin, a non-selective cyclooxygenase inhibitor, reduced acetylcholine-induced relaxation in 2K group, suggesting that prostacyclin is a vasodilator in renal arteries. Indomethacin did not affect acetylcholine-induced relaxation in 1K mice, but imp...