Study On Mechanisms Of Action Of Liuwei Dihuang Decoction With Proteomic And Metabonomic Techniques

Objective : Liuwei Dihuang (LW) decoction are classical traditional Chinese medicinal prescription for anti‐aging. Senescence‐accelerated mouse (SAM) is a good model to study mechanisms of aging. To better understand the mechanisms of actions of LW on antiaging, systematic techniques, including proteomic and metabonomic methods, were employed in this study. Method : SAM‐prone/8 (SAMP8) were orally administered with LW for consecutive 30 days. Two dimensional electrophoresis (2‐DE) based proteomic technique and 1 H nuclear‐magnetic‐resonance‐spectroscopy (NMR) based metabonomic technique were employed to investigate the proteins and metabolites that LW acted on. Results: The expressions of serum proteins in SAMP8 before and after LW treatment were quite different. There were 21 differentially expressed proteins after LW treatment, which were related to the functions of transcriptional control, cytoskeleton, cell signaling, etc.. Principle components analysis of 1 HNMR spectra showed clear separation between serum samples of SAMP8 before and after LW treatment. Metabolites that LW affected were glucose, lactate, high density lipoprotein, etc. related to glucose, lipids, lipoproteins and amino acids metabolism. Conclusion: These findings suggested abnormal serum protein expressions and metabolism disequilibrium in SAMP8 caused by aging and the mechanisms of actions of the LW may lie in restoring of the normal condition of specific proteins and metabolism equilibrium in SAMP8. Grant Funding Source : Supported by grants from the National Natural Science Foundation of China 30701073, 90709012