Comparison of two methods for the isolation of phytolith occluded carbon from plant material

Background and aims Phytolith occluded carbon (PhytOC) is of interest for isotope studies, dating of sediments and the capture and storage of carbon. Many methodologies have been used for the isolation of phytoliths from plant material; however, there are wide disparities in the PhytOC contents when determined by different methodologies. In this study we examine the utility of the two main methods used for quantifying PhytOC. Methods These methods are: (1) a microwave digestion followed by a Walkley-Black digestion, and (2) H 2 SO 4 /H 2 O 2 . Results Method (1) produced PhytOC values over 50 times higher than those acquired by method (2). SEM examination indicated that the differences were likely due to shattering of the phytoliths by method (2) allowing consumption by the acid and peroxide of PhytOC . Conclusion These results indicate that for the samples analysed here: 1] the modified microwave method allowed the total PhytOC to be measured, 2] the H 2 SO 4 /H 2 O 2 method allowed the PhytOC within the tightly packed silica matrix to be measured, and 3] the PhytOC retained within the phytolith cavities could possibly be calculated by subtracting 2] from 1]. For the samples analysed here most of the PhytOC resided in the phytolith cavities.