Flavobacterium buctense sp. nov., isolated from freshwater

A gram-negative, non-gliding motile, aerobic bacterium, designated as strain T7 T , was isolated from freshwater of Chishui River flowing through Maotai town, Guizhou Province, southwest of China. Based on the 16S rRNA gene sequence analysis, the isolate was identified as a member of the genus Flavobacterium and that shared less than 97 % sequence similarities with recognized Flavobacterium species. Its closest phylogenetic relative was Flavobacterium dankookense (96.9 %), followed by Flavobacterium cheonhonense (96.8 %) and Flavobacterium macrobrachii (96.7 %). The strain formed smooth yellow colonies on R2A plates, and cells were observed to be short rods. Strain T7 T was found to be able to grow at 15–30 °C (optimum 25 °C), at NaCl concentration of 0–0.5 % (optimum 0 %) and at pH 6.5–9.5 (optimum pH 7.5). Catalase and oxidase tests were positive. Polar lipids of strain T7 T included phosphatidylethanolamine, four unidentified polar lipids, one unidentified phospholipid and one unidentified aminolipid. Chemotaxonomic analysis revealed menaquinone-6 as the dominant respiratory quinone and C 15:0 , iso-C 15:0 and iso-C 15:1 as the major fatty acids. The DNA G+C content of strain T7 T was determined to be 38.2 mol%. On the basis of phylogenetic, phenotypic and genetic data obtained in this study, strain T7 T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium buctense sp. nov. is proposed. The type strain is T7 T (=JCM 30750=CGMCC 1.15216).