Validation Of Isolation Methodology And Endogenous Control Selection For Qrt-Pcr Assessment Of Microrna Expression In Serum And Urine Exosomes
BLOOD(2014)
摘要
Introduction: MicroRNAs are short RNA molecules that control ~50% of genes by binding to the mRNA 3’ UTR and repressing translation. Recently, they have been detected within exosomes; small vesicles secreted by most cells and abundant in body fluids. Exosomes are highly enriched for specific microRNAs and have been proposed as the starting point for circulating biomarker studies.
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关键词
microrna expression,exosomes,qrt-pcr
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