Size fractionation and size characterization of nanoemulsions of lipid droplets and large unilamellar lipid vesicles by asymmetric-flow field-flow fractionation/multi-angle light scattering and dynamic light scattering

•Lipid droplets and lipid vesicles of different chemical composition were prepared.•Size characteristics of lipid particles were determined by AF4-MALS, DLS and TEM.•Shape and internal structure of lipid particles was inferred from the shape factor.•Size dispersity of lipid vesicles containing higher cholesterol amount was broader.•Lipid vesicles showed narrower size distribution than lipid droplets.