Registration of ‘SY Clearstone 2CL’ Wheat

'SY Clearstone 2CL' (Reg. No. CV-1094, PI 668090) hard red winter wheat (Triticum aestivum L.) was developed by the Montana Agricultural Experiment Station and released in September 2012 through a marketing agreement with Syngenta Seeds. SY Clearstone 2CL is a two-gene Clearfield backcross-derivative of 'Yellowstone' (PI 643428) hard red winter wheat with resistance to imazamox herbicide Beyond (BASF Corporation). SY Clearstone 2CL was selected from the cross Yellowstone*4/3/MTCL01158/Teal 11A//'Jagalene' (PI 631376). Yellowstone is a high-yielding hard red winter wheat released by the Montana Agricultural Experiment Station in 2006. MTCL01158 is an unreleased doubled haploid breeding line [TX12588*4/FS2//Tiber (PI 517194)], which served as the source of proprietary mutant allele TaAHASL1D. Proprietary hard red spring wheat germplasm line Teal 11A (BASF Corporation) served as the source of mutant gene TaAHASL1B. Alleles for herbicide resistance were selected during the backcross process using single-nucleotide, allele-specific polymerase chain reaction markers for TaAHASL1D and TaAHASL1B developed by BASF. SY Clearstone 2CL was selected as an F-2:4 line in 2009 following three generations of screening of the backcross population with imazamox herbicide. Results of three years of replicated field testing indicate that SY Clearstone 2CL is similar to recurrent parent Yellowstone for grain yield, most agronomic characteristics, and milling and baking qualities.