Plasma Polymerization of Amines and Acetone: Functionalized Surfaces for Microarray Applications

Plasma polymerization ( PP) afforded amine as well as carbonyl containing films which are suitable for use in microarray analysis. The polymerization process with acetone (AC), ethylenediamine (EDA) and diethylamine (DEA) has to be performed in the presence of argon as a carrier gas to be successful. Different activation steps of the polymer based support yield surfaces which differ significantly in their elemental composition after the PP step. Angle dependent XPS proves a take off angle dependence of the carbonyl PP film in contrast to a homogeneous PP film for amine functionalized PP layers while microarray experiments have been successful for both functional films.