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A simple method for differentiation of H9 cells into neuroectoderm

Annuo Liu,Dijuan Zhang,Lihua Liu,Juan Gong,Chao Liu

Tissue and Cell（2015）

Cited 4|Views8
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Abstract
•Disassociated H9 single cells were reaggregated into small cell clusters.•The cell clusters formed NE cells by NE induction.•Expression of PAX6 mRNA was detected in NE-derived cells.•The beta-III-tubulin positive neurons were generated from NE cells.•Coexpression of beta-III-tubulin and TH was detected in NE-derived cells.
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ESC,NE,RT-PCR,IF,IB,TH,DA,PD,LCD,AA,DAPI,FGF
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