A simple method for differentiation of H9 cells into neuroectoderm
Tissue and Cell(2015)
Abstract
•Disassociated H9 single cells were reaggregated into small cell clusters.•The cell clusters formed NE cells by NE induction.•Expression of PAX6 mRNA was detected in NE-derived cells.•The beta-III-tubulin positive neurons were generated from NE cells.•Coexpression of beta-III-tubulin and TH was detected in NE-derived cells.
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Key words
ESC,NE,RT-PCR,IF,IB,TH,DA,PD,LCD,AA,DAPI,FGF
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