Biodiversity of microbial consortia isolated from traditional fresh sheep cheese Karakačanski skakutanac

The aim of this study was to assess the structure of indigenous microbial community associated with traditional fresh sheep cheese Karakacanski skakutanac and to preserve autochthonous microbial consortia. Eleven cheeses were sampled during production season (April-September) and subjected to microbiological analysis. Bacterial DNA was isolated by Maxwell (R) 16 DNA system from 99 microbial consortia harvested from three culture media (M17, Rogosa, CATC) on the 1(st), 2(nd) and 3(rd) day of the cheese shelf life. The extracted bulk DNA (n = 99) was used as a template for PCR-ARDRA and PCR-DGGE analysis. There were no dramatic shifts in the bacterial number and structure of the microbial consortia harvested on the 1(st), 2(nd) or 3(rd) day of the cheese shelf life neither during period of sampling. Lactococcus lactis subsp. lactis reached the number of 10(7)-10(8) CFU g(-1), while Leuconostoc pseudomesenteroides, Enterococcus faecalis, and Lactobacillus versmoldensis were identified only at lower dilutions (10(-2) - 10(-3)). This first polyphasic microbiological-molecular study of the Karakacanski skakutanac indicated the main LAB representatives associated with the cheese. Obtained autochthonous microbial consortia present a valuable pool of strains for further genetic and functional characterizations.