Emergence of a novel GyrA variant from a strain of multidrug-resistant Klebsiella pneumoniae isolated from in China

A strain of Klebsiella pneumoniae (NB029) was positive for blaTEM (beta-lactamase gene), aph(3')-I aminoglycoside modifying enzyme gene), gyrA (quinolone resistance gene), trbC (genetic marker of plasmids), tnp513, IS26, IS903 (genetic markers of transposons and insertion sequences), int I 1 (genetic marker of integrons), qacE Delta 1 (drug efflux gene) by PCR assays. So these positive genes confer multidrug-resistant phenotype. And the presence of gyrA, confirmed as a novel variant (Genbank accession number: JN232083), was determined by sequencing and Blastsguin algorithm. The double mutations at codon 83 and codon 87 in the quinolone resistance-determining region of the gyrA gene lead to high level quinolone resistance.