Semen conservation and seminal clot liquefaction of capuchin monkey (Cebus apella) in powdered coconut water extender (PCW) at different temperatures

The aim of this study was to evaluate the powdered coconut water (PCW) in the semen conservation and seminal clot liquefaction. The semen of six adult male Cebus apella was collected by electroejaculation (EEJ), diluted in ACP-118 (R) extender and stayed in water bath at 33, 35 and 37 degrees C for 24 hours. The sperm integrity was evaluated by eosin-nigrosine staining every one hour during the six initial hours and after 24 hours of incubation. The average volumes and sperm concentrations of clotted and liquid fractions were 0.20 +/- 0.02 and 0.20 +/- 0.10mL, 1.1 +/- 0.3x10(8) and 1.3 +/- 0.9x10(7) sperm mL(-1). respectively. Immediately after collection, only, in a sample of liquid fraction was observed 20% motility and vigor 4, which stopped after 40 Minutes. Most of the clot was liquefied in ACP-118 (R) after 12 hours of incubation. The best observed treatment was 33 degrees C, because it kept 47 +/- 12.8% of sperm integrity after 24 hours. It was concluded that the PCW extender is effective in the liquefaction of seminal clot and maintenance of sperm viability 24 hours after the EEJ at 33, 35 and 37 degrees C.