An ELISA using recombinant TmHSP70 for the diagnosis of Taenia multiceps infections in goats
Veterinary Parasitology(2015)
Abstract
•We develop a novel ELISA for detecting T. multiceps infections in goats using TmHSP70.•The TmHSP70 gene was successfully amplified, cloned and expressed in BL21(DE3).•The recombinant TmHSP70 protein exhibited good immunogenic properties.•The TmHSP-based ELISA exhibited high sensitivity and specificity.•The TmHSP-based ELISA is a novel tool for the rapid diagnosis of coenurosis in goats.
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Key words
Tapeworm,Taenia multiceps,TmHSP 70,Clone,Expression,Immunodiagnosis,Indirect ELISA
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