Simultaneous Spectrophotometric Determination of Isoperoxisomicine A1 and Peroxisomicine A1 Using Partial Least Square Regression Type 1 (PLS-1)

Recibido el 26 de julio del 2004; aceptado el 10 de diciembre del 2004 Abstract. Multivariate calibration with regression by partial least squares (PLS-1) is herein proposed as an alternative method for the spectrophotometric quantification of isoperoxisomicine A1 in peroxi- somicine A1 batches. In order to minimize the optimal factors neces- sary to obtain the calibration matrix, different parameters were evalu- ated. The adequate selection of the spectral regions proved to be important on the number of factors. In order to simultaneously quan- tify both analytes, the spectral region between 252 and 340 nm was selected. Recoveries for isoPA1 and PA1 were 97% and 103% respectively. The developed method was applied to two batches of PA1; the amount of isoPA1 found, showed no significant differences with those found by means of 1 H RMN.