Proposal Of Nine Novel Species Of The Genus Lysinimicrobium And Emended Description Of The Genus Lysinimicrobium

Thirteen novel Gram-stain-positive bacteria were isolated from various samples collected from mangrove forests in Japan, and their taxonomic positions were investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequence comparisons showed that the 13 isolates formed a single Glade with Lysinimicrobium mangrovi HI08-69(T), with a similarity range of 97.6-99.5 %. The peptidoglycan of the isolates was of the A4 alpha type with an interpeptide bridge comprising Ser-Glu and an L-Ser residue at position 1 of the peptide subunit. The predominant menaquinone was demethylmenaquinone DMK-9(H-4) and the major fatty acid was anteiso-C-15 :0.These chemotaxonomic characteristics corresponded to those of the genus Lysinimicrobium. On the basis of the phenotypic and phylogenetic data, along with average nucleotide identity values among the isolates, we concluded that the 13 isolates should be assigned to the following nine novel species of the genus Lysinimicrobium: Lysinimicrobium aestuarii sp. nov. (type strain HI12-104(T)=NBRC 109392(T)=DSM 28144(T)), Lysinimicrobium flavum sp. nov. (type strain HI12-45(T)=NBRC 109391(T)=DSM 28150(T)), Lysinimicrobium gelatinilyticum sp. nov. (type strain HI12-44(T)=NBRC 109390(T)=DSM 28149(T)), Lysinimicrobium iriomotense sp. nov. (type strain HI12-143(T)=NBRC 109399(T)=DSM 28146(T)), Lysinimicrobium luteum sp. nov. (type strain HI12-123(T)=NBRC 109395(T)=DSM 28147(T)), Lysinimicrobium pelophilum sp. nov. (type strain HI12-111(T)=NBRC 109393(T)= DSM 28148T), Lysinimicrobium rhizosphaerae sp. nov. (type strain HI12-135(T)=NBRC 109397(T)=DSM 28152(T)), Lysinimicrobium soli sp. nov. (type strain H112-122(T)=NBRC 109394(T)=DSM 28151(T)) and Lysinimicrobium subtropicum sp. nov. (type strain HI12128(T)=NBRC 109396(T)=DSM 28145(T)). In addition, an emended description of the genus Lysinimicrobium is proposed.