Tumebacillus Lipolyticus Sp Nov., Isolated From River Water

An aerobic, endospore-forming, alkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain NIO-S10(T), was isolated from a surface water sample collected from the Godavari River, Kapileswarapuram, India. Colonies on nutrient agar were circular, 3-4 mm in diameter, creamish and raised after incubation for 36 h at 37 degrees C. Growth occurred at 20-40 degrees C, at pH 6-9 and in the presence of 0-2 % (w/v) NaCI. Strain NIO-S10(T) was positive for oxidase, caseinase, DNase, gelatinase, lipase and urease activities, and negative for catalase, aesculinase, amylase and cellulase activities. The fatty acids were dominated by branched and saturated fatty acids, with a high abundance of iso-C-15 : 0, anteiso-C-15 : 0 and C-18 : 0.The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid, and MK-7 was the major menaquinone. The DNA G+C content of strain NIO-S10(T) was 54.4 mol%. A BLAST sequence similarity search based on 16S rRNA gene sequences indicated that Tumebacillus ginsengisoli Gsoil 1105(T) was the nearest phylogenetic neighbour to strain NIO-S10(T), with a pairwise sequence similarity of 94.9 %. Phylogenetic analysis showed that strain NIO-S10(T) was clustered with members of the genus Tumebacillus and together with members of the genus Effusibacillus. Based on phenotypic characteristics and phylogenetic inference, strain NIO-S10(T) represents a novel species of the genus Tumebacillus, for which the name Tumebacillus lipolyticus sp. nov. is proposed. The type strain is NIO-S10(T) (=KCTC 32289(T)=MTCC 12483(T)).