Identification and testing of reference genes for gene expression analysis in pollen of Pyrus bretschneideri

Jianqing Chen, Xinyue Li,Danqi Wang,Leiting Li,Hongsheng Zhou,Zhe Liu,Jun Wu,Peng Wang,Xueting Jiang,Musana R Fabrice,Shaoling Zhang

Scientia Horticulturae（2015）

引用 33|浏览16

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摘要

•Validate optimal reference genes for qRT-PCR in pear pollen.•Develop tree reference genes to detect the purity of cDNA.•Identify the expression pattern of PbrVHA-a in pear pollen.

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关键词

ABA,ACT,APT,Ct,CYP,EF1α,GAPDH,PP2A,qRT-PCR,R,TUB,UBQ,UBI,V-ATPase,VHA-a,18S rRNA

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