Identification and testing of reference genes for gene expression analysis in pollen of Pyrus bretschneideri
Scientia Horticulturae(2015)
摘要
•Validate optimal reference genes for qRT-PCR in pear pollen.•Develop tree reference genes to detect the purity of cDNA.•Identify the expression pattern of PbrVHA-a in pear pollen.
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关键词
ABA,ACT,APT,Ct,CYP,EF1α,GAPDH,PP2A,qRT-PCR,R,TUB,UBQ,UBI,V-ATPase,VHA-a,18S rRNA
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