COMPARATIVE ASSESSMENT OF GENETIC DIVERSITY IN WILD AND CULTIVATED BARLEY USING RIBOSOMAL DNA SPACER LENGTH VARIANTS

J Ramamoorthy, Daniel K Chong,C C Chinnappa

Israel Journal of Plant Sciences(2013)

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Abstract
Ribosomal DNA spacer length variants (slv) were used to compare the genetic diversity between Hordeum spontaneum, the supposed progenitor of cultivated barley, and landraces of barley, H. vulgare, collected from three countries in the eastern Mediterranean region. A total of six rDNA slv and six slv phenotypes were observed. All six slv and phenotypes were found in both species. Nei's heterozygosity index was higher for landraces in all three countries. Landraces possessed slightly higher diversity than H. spontaneum. Slv phenotype 1 (112, 104) was the most predominant type in both species, and the frequency distributions for the two species were similar. This suggests that H. vulgare and H. spontaneum belong to the same polymorphic species. Furthermore, H. spontaneum seen today in the eastern Mediterranean region might have been derived from an extinct two-rowed ancestor.
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Key words
cultivated barley,genetic diversity
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