Efficacy of Acetone and Ethanol in Dehydrating Canine Body Sheets :Before and After Resin Coating

The aim of this study was to compare the efficacy of ethanol and acetone in dehydration of canine body sheets before preservation in resin for a 16-week period. A 10-kg male dog which died of the respiratory failure was preserved in 10% formalin via common carotid artery at room temperature for 7 days prior to being kept in a freezer at -25 degrees C for 7 days. The body of the dog was cut into 2.5-cm-thick sheets with a band saw. The body sheets were separately dehydrated for 3 days with either acetone or ethanol. In this study, the color of muscles and fat from acetone-dehydrated sheets was more significantly distinct than those dehydrated by ethanol, while the color of the organ surface was slightly changed in both groups. The shrinkage of body sheets did not significantly differ between the two groups. After dehydration, the body sheets were subject to preservation in resin for 16 weeks. At the first day in resin, the color of organs was slightly changed. After 16 weeks, the transparency of resin was not changed and the shrinkage of body sheets was not significantly different in body sheets of both groups.