Reduced Concentration Of Androstenone And Up-Regulation Of 3 Beta-Hydroxysteroid Dehydrogenase And 17 Beta-Hydroxysteroid Dehydrogenase Mrna Levels By Active Immunisation Against Gonadotropin Releasing Hormone I

The aim of this study was to investigate the efficacy of active immunisation against recombinant maltose binding protein-gonadotropin releasing hormone I (MBP-GnRH-I6) in preventing boar taint. The concentrations of testosterone and anti-GnRH-I antibodies in serum were determined by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The mRNA levels of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) in testes were analysed by real time quantitative PCR. The concentrations of androstenone and 3-methylindole in backfat were assayed by HPLC. Active immunisation against MBP-GnRH-I6 increased the serum concentration of anti-GnRH-I antibodies (P<0.05) and the levels of 3 beta-HSD and 17 beta-HSD mRNA in testis. However, it reduced the serum concentrations of testosterone (P<0.05) and androstenone. The concentration of 3-methylindole in backfat (P>0.05) was not changed. Thus, we conclude that vaccination against MBP-GnRH- I6 is a practical and effective method to suppress the synthesis of androstenone.