Selenium speciation in soybean by high performance liquid chromatography coupled to electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS/MS)
Microchemical Journal(2015)
摘要
Selenium enriched soybean was cultivated in experimental fields for 4months in which the manure-based growth medium contained different concentrations (0, 50, 100, 150, 200mg/kg) of sodium selenite. Total selenium in the soybean increased across the different concentrations of selenium used, reaching a concentration of 1118.0μg/kg at the maximum concentration of selenium used. The concentration of Se-protein in the soybean was 447.2μg/kg. The speciation of selenium in the soluble fraction of the soybean protein was determined by enzymatic hydrolysis, followed by high performance liquid chromatography coupled to electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS/MS), performed under optimized parameters. Se-methyselenocysteine (Se-MeSeCys) and selenomethionine (Se-Met) were monitored by selecting the multiple reaction monitoring (MRM) mode. The results showed that inorganic selenium was absorbed by the soybean and biotransformed mainly into Se-MeSeCys. Se-MeSeCys represented 66.4% of the selenium in Se-protein, and 29.2% of the total selenium in the soybean. The result showed that HPLC–ESI–MS/MS was a sensitive and accurate method for the identification and quantification for the speciation of selenium in soybean.
更多查看译文
关键词
HPLC–ESI–MS/MS,Se-enriched soybean,Speciation
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要