Comparison of the alpha-amylase inhibitor-1 from common beans and transgenic pea expressing the bean alpha-amylase inhibitor-1 by means of LC–TOF–MS

Recently, more attention is given to legume seed derived alpha amylase inhibitors (αAI) as it seems they effectively can be applied in crop protection against attack by insect larvae and prevention of obesity, weight control or diabetes type 2 diseases. Several researches have described the structure-function relationship of bean αAI-1. Some heterogeneous structural variations were revealed in their biologically active α− and β-chains due to the different amino acid composition and glycan patterns. However, further accumulation of the data is still required for the variability assessment of the bean αAIs and its transgenic expression.