Micro-Spectrophotometry Applied To Intracellular Ph Determination

H Sun,F Jacquey, Jc Bernengo

PROCEEDINGS OF THE ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOL 14, PTS 1-7（1992）

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Abstract

This paper presents intra-cellular pH measurements, performed through absorbance technique. Calibration data on rat myoballs have been obtained with a specially designed micro-spectro- photometer. Dye-protein interactions have been investigated, and dye titration curves inside living cells are presented. Absolute cytoplasmic pH determination are shown to be possible with an accuracy of 0.02 pH unit.

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Key words

biochemistry,molecular biophysics,accuracy,calibration,proteins,biology,intracellular ph,ph

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