Effect of genetic background and postinfectious stress on visceral sensitivity in Citrobacter rodentium-infected mice.
NEUROGASTROENTEROLOGY AND MOTILITY(2016)
摘要
Background Infectious gastroenteritis is a major risk factor to develop postinfectious irritable bowel syndrome (PI-IBS). It remains unknown why only a subgroup of infected individuals develops PI-IBS. We hypothesize that immunogenetic predisposition is an important risk factor. Hence, we studied the effect of Citrobacter rodentium infection on visceral sensitivity in Th1-predominant C57BL/6 and Th2-predominant Balb/c mice. Methods Eight-week-old mice were gavaged with C.rodentium, followed by 1h of water avoidance stress (WAS) at 5weeks PI. At 10, 14days, and 5weeks PI, samples were assessed for histology and inflammatory gene expression by RT-qPCR. Visceral sensitivity was evaluated by visceromotor response recordings (VMR) to colorectal distension. Key Results Citrobacter rodentium evoked a comparable colonic inflammatory response at 14days PI characterized by increased crypt length and upregulation of Th1/Th17 cytokine mRNA levels (p(uncorrected) < 0.05) in both C57BL/6 and Balb/c mice. At 5weeks PI, inflammatory gene mRNA levels returned to baseline in both strains. The VMR was maximal at 14days PI in C57BL/6 (150 +/- 47%; p=0.02) and Balb/c mice (243 +/- 52%; p=0.03). At 3weeks PI, the VMR remained increased in Balb/c (176 +/- 23%; p=0.02), but returned to baseline in C57BL/6 mice. At 5weeks PI, WAS could not re-introduce visceral hypersensitivity (VHS). Conclusions & InferencesCitrobacter rodentium infection induces transient VHS in C57BL/6 and Balb/c mice, which persisted 1week longer in Balb/c mice. Although other strain-related differences may contribute, a Th2 background may represent a risk factor for prolonged PI-VHS. As PI-VHS is transient, other factors are crucial for persistent VHS development as observed in PI-IBS.
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关键词
Citrobacter rodentium,irritable bowel syndrome,visceral hypersensitivity
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