Anxiolytic and sedative activities of aqueous leaf extract of Dichrocephala integrifolia (Asteraceae) in mice.

Ethnopharmacological relevance: Dichrocephala integnfolia (Asteraceae) is commonly used in the folk medicine in Cameroon to treat various ailments including headaches, jaundice, filariasis, neuritis and children's night agitation. It is also known to have anxiolytic properties. Since there were no scientific data supporting the above claims, we therefore aimed to evaluate the anxiolytic and sedative effects of the aqueous leaf extract of D. integrifolia.Materials and methods: The extract used was obtained after maceration of leaves of D. integrifolia in water at room temperature. The anxiolytic effect was tested by the elevated plus-maze (EPM) and Open Field tests while the sedative effect was evaluated by the diazepam-induced sleep test.Results: D. integrifolia significantly induced an increase in the percentage of entries into open arms and a decrease in percentage of entries into closed arms at the dose of 22 mg/kg BW. The extract also induced a decrease of rearing and head dipping at the doses of 22 and 40 mg/kg BW during the EPM test. In addition, D. integnfolia extract induced a significant increase of crossing and time spent at the center of the experimental set at the dose of 40 mg/kg BW during the open field test. Lastly, D. integrifolia caused a significant reduction of the latency to sleep and an increase of total sleep time at the doses of 22 and 40 mg/kg BW in the diazepam-induced sleep test.Conclusion: The results obtained in this study suggest that the aqueous leaf extract of D. integrifolia is endowed with anxiolytic and sedative properties, which justify its traditional use. (C) 2015 Elsevier Ireland Ltd. All rights reserved.