Comamonas Phosphati Sp Nov., Isolated From A Phosphate Mine

A Gram-stain-negative, facultatively anaerobic, non-pigmented, non-sporulating, rod-shaped bacterial strain (WYH 22-41(T)) was isolated from a phosphate mine in Yunnan Province, China. The cells were motile with a single polar flagellum. The 16S rRNA gene of strain WYH 22-41(T) was phylogenetically related to the corresponding gene of Comamonas terrae DSM 27221(T) (98.4 % 16S rRNA gene sequence similarity), Comamonas odontotermitis LMG 23579(T) (97.6 %) and Comamonas aquatica LMG 2370(T) (97.4 %). DNA DNA hybridizations of strain WYH 22-41(T) with these three strains showed relatedness values of 33.2 %, 20.5 % and 27.7 %, respectively. The DNA G+C content of strain WYH 22-41(T) was 62.4 mol%. The predominant respiratory quinone was ubiquinone-8.The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids of strain WYH 22-41(T) were C-16:0, C-17:0 cyclo, summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) and summed feature 8 (C-18:1 omega 6c and/or C-18:1 omega 7c). On the basis of phenotypic properties, phylogenetic characteristics, DNA DNA hybridization, as well as whole-cell fatty acid composition, strain WYH 22-41(T) represents a novel species of the genus Comamonas, for which the name Comamonas phosphati sp. nov. is proposed. The type strain is WYH 22-41(T) (=CGMCC 1.12294(T)=DSM 26017(T)).