MiRNA-381抑制肾癌侵袭能力及其分子机制
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences(2015)
Abstract
目的:研究miRNA-381对肾癌侵袭的抑制作用,并探索其机制.方法:上调miRNA-381表达后,观察miRNA-381对肾癌细胞侵袭的抑制作用.通过miR数据库starBase研究miRNA-381靶基因,再通过双荧光素酶实验验证.分析miRNA-381及相应靶基因在细胞和组织水平的表达丰度.结果:转染miRNA-381后,其表达增加,跨膜细胞数目显著下降,细胞侵袭力受抑制.生物信息学分析显示miRNA-381靶基因为CREB绑定蛋白(CREB binding protein,CBP)、β-catenin和淋巴增强因子-1(lymphoid enhancer binding factor-1,LEF-1);包含野生型靶基因3'-非编码区载体组的荧光素酶活性明显降低.实时定量PCR显示肾癌细胞中miRNA-381低表达,在高转移潜能肾癌细胞株786-OHM中表达更低;相反,在细胞、组织水平miRNA-381靶基因CBP,β-catenin与LEF-1表达均增高.结论:MiRNA-381可抑制肾癌细胞的侵袭能力,其机制可能是通过CBP,β-catenin和LEF-1实现的.
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Key words
miRNA-381,renal cancer,invasion,CREB binding protein,β-catenin,lymphoid enhancer binding factor-1
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