Flavobacterium Paronense Sp Nov., Isolated From Freshwater Of An Artificial Vegetated Island

A Gram-stain-negative, motile by gliding, yellow-pigmented bacterial strain, designated KNUS1(T), was isolated from Lake Paro in Korea. The phylogenetic tree based on 16S rRNA gene sequences showed that strain KNUS1(T) formed a distinct lineage within the genus Flavobacterium. Strain KNUS1(T) was closely related to Flavobacterium cheonhonense ARSA-15(T) (96.8 %16S rRNA gene sequence similarity), Flavobacterium pectinovorum DSM 6368(T) (96.3 %) and Flavobacterium dankookense ARSA-19(T) (96.1 %). The major fatty acids of strain KNUS1(T) were iso-C-15 : 0, and iso-C-15 : 1 G. The major polyamine was sym-homospermidine. The major polar lipids of strain KNUS1(T) were phosphatidylethanolamine, five unidentified aminolipids and three unidentified polar lipids. The major respiratory'quinone was menaquinone 6 (MK-6). The DNA G+C content of strain KNUS1(T) was 34.2 mol%. On the basis of the evidence presented in this study, strain KNUS1(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium paronense sp. nov. is proposed. The type strain is KNUS1(T) (=KACC 17692(T)=CECT 8460(T)).