Effects Of Soothing Liver And Invigorating Spleen Recipes On The Ikk Beta-Nf-Kappa B Signaling Pathway In Kupffer Cells Of Nonalcoholic Steatohepatitis Rats

This study investigates the effect of soothing liver and invigorating spleen recipes on steatohepatitis examining the IKK beta-NF-kappa B signaling pathway in KCs of NASH rats. SD male rats were randomly divided into 8 groups, and the NASH model was induced by a high-fat diet (HFD). After 26 weeks, liver tissue was examined in H&E stained sections and liver function was monitored biochemically. KCs were isolated by Seglen's method, with some modifications. The mRNA and protein expression of the IKK beta-NF-kappa B signaling pathway components was examined by quantitative PCR and Western blotting. The results show that the high-fat diet induced NASH in the rats, and the soothing liver recipe and invigorating spleen recipe decreased the levels of TNF-alpha, IL-1, and IL-6 in KCs, as well as inhibiting the mRNA and protein expression of the IKK beta-NF-kappa B signaling pathway components. In conclusion, the experiment indicated the importance of the IKK beta-NF-kappa B signaling pathway in KCs for the anti-inflammatory effects of the soothing liver and invigorating spleen recipes.