iSMS: single-molecule FRET microscopy software
NATURE METHODS（2015）
Abstract
To the Editor: Single-molecule fluorescence resonance energy transfer (smFRET) with total-internal-reflection fluorescence (TIRF) microscopy is a powerful tool for studying the structure and conformational dynamics of biomolecules1, 2, 3. By reporting on nanoscale distances within single biomolecules, this technique provides the means to uncover population heterogeneity that is normally hidden behind… Download references
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Key words
Single-molecule biophysics, Total internal reflection microscopy, Image processing, Fluorescence imaging
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