Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

Anca M Paşca,Steven A Sloan,Laura E Clarke,Yuan Tian,Christopher D Makinson,Nina Huber,Chul Hoon Kim,Jin-Young Park, Nancy A O'Rourke,Khoa D Nguyen,Stephen J Smith,John R Huguenard,Daniel H Geschwind,Ben A Barres,Sergiu P Paşca

Nature methods（2015）

引用 1088|浏览38

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摘要

method for 3D differentiation of human pluripotent stem cells yields brain cortical spheroids with functional neurons and astrocytes. The spheroids can be sliced for imaging and electrophysiological studies.

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关键词

Cellular neuroscience

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