Post-translational modification of a chimeric EPO-Fc hormone is more important than its molecular size in defining its in vivo hematopoietic activity.

•Mammary gland adenoviral transduction is a robust methodology for protein expression.•A sialylated N-glycans profile defines the hematopoietic activity of EPO-Fc variants.•Goat mammary cells aren't a proper system for the active glyco-hormones expression.