载脂蛋白E基因敲除小鼠脑部TNF-α和IL-1β及IL-8的水平升高
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology(2015)
Abstract
目的 探讨载脂蛋白E基因敲除(ApoE--)后小鼠脑部海马区和皮质区肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1 β)及IL-8表达变化.方法 4周龄野生型和ApoE-/-雄性小鼠各10只,饲养12周后断颈处死.采集血液样本进行血脂水平检测;将脑组织固定后切片,HE染色观察形态学改变;免疫组织化学染色检测TNF-α、IL-1β、IL-8蛋白表达.结果 IL-1β和IL-8在ApoE-/-小鼠海马区和皮质区表达的阳性细胞数均显著高于野生型小鼠.TNF-α在ApoE-/-小鼠皮质区表达的阳性细胞数显著高于野生型小鼠,ApoE-/-小鼠海马区TNF-α的表达强度高于野生型小鼠.结论 ApoE-/-小鼠脑部TNF-α、IL-1β、IL-8表达增加.
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Key words
apolipoprotein E knockout,inflammatory factors,brain
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