Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa.

Phillip Ringel,Corinna Probst,Thorben Dammeyer,Sabine Buchmeier,Lothar Jänsch,Josef Wissing,Philip Tinnefeld,Ralf R Mendel,Brigitte M Jockusch,Tobias Kruse

Fungal Genetics and Biology（2015）

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摘要

•The Strep-tag® system was adapted to Neurospora crassa.•Anti-Strep-tag® antibodies have been developed.•Strep-tagged® nitrate reductase was purified from N. crassa and characterized.

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关键词

Neurospora crassa,Nitrate reductase,Recombinant protein expression,Strep-tag®,Post-translational modification

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