Enzymatic production of biodiesel from Nannochloropsis gaditana lipids: Influence of operational variables and polar lipid content.

•Saponifiable lipids (SLs) were extracted from wet microalgal biomass.•SLs were transformed to biodiesel by lipase catalyzed transesterification.•The importance of polar lipids and SL purity for biodiesel conversion is showed.•Influence of solvent and methanol/SL ratio on lipase stability is discussed.•94.7% of microalgal SLs were transformed to biodiesel in the optimized conditions.