Pfge For Shiga Toxin-Producing Escherichia Coli 0157:H7 (Stec 0157) And Non-0157 Stec

This chapter describes the procedure of generating pulsed-field gel electrophoresis (PFGE) profiles (DNA fingerprints) of Shiga toxin-producing Escherichia coli 0157:H7 (STEC 0157) and non-0157 STEC strains within 48 h, based on the standardized laboratory protocol developed by the Centers for Disease Control and Prevention, USA. The protocol describes the preparation of agarose plugs containing STEC 0157 and non-0157 STEC cells, the digestion of bacterial DNA in the plugs using restriction endonuclease enzymes, and the electrophoresis conditions to generate the characteristic PFGE profiles of STEC 0157 and non-0157 STEC isolates.