Determination of iohexol in human serum by a semi-automated liquid chromatography tandem mass spectrometry method.

•Iohexol clearance is an accurate way to measure glomerular filtration rate in children.•Iohexol is quantified in 50μL serum by LC-MS/MS using ioversol as an internal standard.•Sample preparation may be automated using a multi-channel pipetting workstation.