A multi-locus analysis of phylogenetic relationships within grass subfamily Pooideae (Poaceae) inferred from sequences of nuclear single copy gene regions compared with plastid DNA.

•The first phylogeny of grass subfamily Pooideae based on nuclear single copy loci.•Parts of the nuclear genes Acc1, PhyB and Topo6 were used.•The best resolved tree is obtained from combined nuclear DNA data.•Major lineages are supported, except for tribes Diarrheneae, Duthieeae and Stipeae.•Stipoid Ampelodesmos mauritanicus and Trikeraia pappiformis may have hybrid origin.