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Recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibody to turkey coronavirus.

Mohamed Abdelwahab,Chien Chang Loa,Ching Ching Wu,Tsang Long Lin

Journal of Virological Methods（2015）

Cited 26|Views5
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Abstract
•Recombinant TCoV N protein was efficiently produced by a prokaryotic expression system.•An antibody-capture ELISA was established with the recombinant N protein as coating antigen.•Recombinant N protein-based ELISA is more sensitive than IBV-based ELISA for anti-TCoV antibody.•The N protein-based ELISA can be applied for a rapid diagnosis and effective control of outbreaks.
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BCoV,BSA,ELISA,HRPO,IBV,IFA,Mab,NC,PBS,PC,TCoV,TGEV,TMB
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