Molecular and serological in-herd prevalence of Anaplasma marginale infection in Texas cattle.

•A. marginale infection in-herd prevalence in 11 Texas cattle herds was assessed using RT-qPCR and cELISA.•Both RT-qPCR and cELISA results identified herds with high prevalence in the north Texas Rolling Plains and West Trans-Pecos Desert.•Overall prevalence was significantly higher in cattle in north and west Texas compared to east Texas (p<0.0001 for prevalence differences)•Overall RT-qPCR and cELISA results exhibited 90% agreement (kappa = 0.79).•RT-qPCR detected A. marginale infection up to 15 days before cELISA in experimentally infected blood samples.