Establishing column batch repeatability according to Quality by Design (QbD) principles using modeling software.

•Twelve columns from the same brand name, but different batches were compared.•3D design of experiment was used for comparing column selectivity and performance.•3D resolution space allows establishing better method robustness before validation.•A multivariable design space (MODR) allows flexible routine work with columns.•This procedure allows easy column replacement to retain method robustness.