The relative magnitude of transgene-specific adaptive immune responses induced by human and chimpanzee adenovirus vectors differs between laboratory animals and a target species.

•HAdV-5 (HAdV-C) vectors are more immunogenic than AdC68 or ChAdOx1 (HAdV-E) vectors in mice.•In mice, CD8+ T cell responses peak later, and are more durable after HAdV-5 vaccination.•In cattle, ChAdOx1 is at least as immunogenic as HAdV-5.