Expression and refolding of bioactive α-bungarotoxin V31 in E. coli.
Protein Expression and Purification（2015）
Abstract
•Optimized coding sequences produced rαBtx in E. coli without fusion tags.•Optimized simple protocol produced rαBtx similar to its native counterpart.•Ultrasound used in cell lysis can be detrimental to inclusion bodies if overdosed.
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Key words
α-Bungarotoxin,Recombinant protein expression,nAChR,Protein refolding
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