Derivation of cell-adapted Sacbrood virus (SBV) from the native Korean honeybee.

•Sacbrood virus (SBV), a causative agent of larval death in honeybees.•We investigated the growth and replication of SBV in cell cultures.•SBV showed replication signals in mammalian cell lines, including Vero cells, without any signs of cytopathic effects.•SBV can replicate in mammalian cells with sequential passaging after cell adaptation.