Thermodynamic signatures of fragment binding: Validation of direct versus displacement ITC titrations.

Eggert Rühmann,Michael Betz,Marie Fricke,Andreas Heine,Martina Schäfer,Gerhard Klebe

Biochimica et Biophysica Acta (BBA) - General Subjects（2015）

引用 37|浏览9

暂无评分

摘要

•Characterization of the thermodynamic signature of fragment–protein binding•Comparison of direct low-c value titrations with displacement titrations•Enthalpic contribution depends on chemical nature of displacement ligand.•Protocol for the titration of weak binding fragments•Estimation of errors and accuracy of isothermal titration calorimetry of fragments

查看译文

关键词

Thermodynamic signature,Isothermal titration calorimetry,Protein–fragment binding,Displacement titrations,Low-c value titration,Thrombin

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要