Characterization of the N-acetylneuraminic acid synthase (NeuB) from the psychrophilic fish pathogen Moritella viscosa.

Carbohydrate Research(2015)

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Abstract
•The psychrophilic fish pathogenic bacterium Moritella viscosa produces sialic acids.•Presence of sialic acids among fish pathogens points toward an important function.•The N-acetylneuraminic acid synthase NeuB from M. viscosa was characterized.•The enzyme shows typical cold adapted features.•This efficient enzyme can be further explored for production of sialic acids and derivates.
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Sias,Neu5Ac,PSA,LPS,LOS,NeuB,ManNAc,PEP,UDP-N-acetylglucosamine,IPTG,TBA,SDS–PAGE,β-ME,DSC,PSIPRED,DMB-HPLC,MS/MS,RT,+cESI,+pESI,FTMS,MS,ITMS,KM,kcat,CMP,Neu5,7Ac2,Neu,Leg,Pse,HGT,GC,DNA,NaCl,dNTP,EDTA,PNP
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