BSA-stabilized Pt nanozyme for peroxidase mimetics and its application on colorimetric detection of mercury(II) ions.
Biosensors and Bioelectronics(2015)
Abstract
Bovine serum albumin (BSA) is chosen as the nucleation templates to synthesize Pt-based peroxidase nanomimetics with the average diameter of 2.0nm. The efficient Pt nanozymes consist of 57% Pt0 and 43% Pt2+, which possess highly peroxidase-like activity with the Km values of 0.119mM and 41.8mM toward 3,3′,5,5′-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2), respectively. Interestingly, Hg2+ is able to down-regulate the enzymatic activity of Pt nanoparticles, mainly through the interactions between Hg2+ and Pt0. It is the first report to explore a colorimetric Hg2+ sensing system on the basis of peroxidase mimicking activities of Pt nanoparticles. One of our most intriguing results is that BSA-stabilized Pt nanozymes demonstrate the ability to sense Hg2+ ions in aqueous solution without significant interference from other metal ions. The Hg2+ detection limit of 7.2nM is achieved with a linear response range of 0–120nM, and the developed sensing system is potentially applicable for quantitative determination of Hg2+ in drinking water.
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Key words
Protein,Platinum,Nanoparticle,Nanozyme,Hg2+ detection
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