Bi-ligand surfaces with oriented and patterned protein for real-time tracking of cell migration.

Varadraj N Vernekar,Charles S Wallace,Mina Wu,Joshua T Chao,Shannon K O'Connor,Aimee Raleigh,Xiaji Liu,Jason M Haugh,William M Reichert

Colloids and Surfaces B: Biointerfaces（2014）

Cited 5|Views3

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Abstract

•A bioactive platform for detailed observation of 2-D cell migration is presented.•Migration factors are oriented on surface via His- and Fc-tags and IMAC chemistry.•ICAM-1 surface immobilization along with activation enabled B cell migration.•No influence of surface immobilized SDF-1α on activated B cell migration was seen.•Controlled exposure to UV enabled patterning via linear protein gradient fabrication.

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Key words

Chemotaxis,Chemokine,Cell adhesion molecule,Surface immobilization,Surface gradients,B lymphocyte

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