Dereplication of depsides from the lichen Pseudevernia furfuracea by centrifugal partition chromatography combined to 13C nuclear magnetic resonance pattern recognition.
Analytica Chimica Acta(2014)
Abstract
•The major depsides of a lichen extract were directly identified within mixtures.•The initial extract was rapidly fractionated by CPC in the pH-zone refining mode.•Hierarchical clustering of 13C NMR signals resulted in the identification of depside molecular skeletons.•13C chemical shift clusters were assigned to structures using a 13C NMR database.•Six depsides were unambiguously identified by this approach.
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Key words
Centrifugal partition chromatography,13C nuclear magnetic resonance,Hierarchical clustering analysis,Dereplication,Depside,Lichen
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